Transregio SFB-TR 84 “Innate Immunity of the Lung: Mechanisms of Pathogen Attack and Host Defence in Pneumonia“

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Z1a-Subproject

High-end microscopy and digital image analysis in cellular microbiology of the lung (Hocke)

 

Advanced imaging such as spectral confocal, life-cell,-tissue, or super-resolution microscopy, combined with digital image analysis are indispensable for uncovering innate immune mechanisms characterizing the pathogen-host interaction in the pulmonary compartment.

Therefore, this central service project provides a comprehensive microscopic environment starting from protocol establishment, sample preparation, education, and cloning up to configuration of adequate imaging procedures to fulfil the individual needs of the SFB projects. This is additionally supported by a continuous introduction of new imaging procedures such as spectral fluorescence life time imaging or machine learning-based image analysis. By this and due to a close collaboration with industrial partners the infrastructure of Z01a will be successfully transferred into a permanent “Development Centre for Multidimensional Imaging” beyond the third funding period.